Central Microscopy Imaging Center (CMIC)

***The research activities at SBU currently are in the stage of "Research Phase 4" (60%-80% occupancy). Accordingly, the CMIC Facility is open on a part-time basis. Some limitations and additional procedures are adopted at this time. Please see details on iLab calendar for each instrument. Thank you for your support and cooperation.***

 

C-MIC TEM FACILITY

*The C-MIC Facility is in Phase 4 of operation and is open on a part-time basis.  The TEM Facility is closed to all users for imaging on the TEM.  There is no scope training for new users and all imaging will be done by Susan Van Horn.  Please contact Sue to coordinate schedules to drop off your samples.

**Sample preparations (embedding, sectioning,etc.) are still available.  Please contact Susan Van Horn to corrdinate schedules.  There will be no unannounced drop off of samples - you MUST schedule a time to drop off your samples.  Contact Sue for info on how to prepare your samples before you drop them off.

***Masks and gloves must be worn at all times when entering the C-MIC Facility and Sue's lab/office (rooms 048 and 046).

****Please contact Sue 631-632-8623 to schedule and for any questions.

Thank you.

 

Multiphoton Imaging Facility---Light Microscopy

*** The research activities at SBU currently are in the stage of "Research Phase 4" (60%-80% occupancy). Accordingly, the facility now opens ONLY from Monday to Thursday between 9:00 am - 5:00 pm each week. New user trainings in-person are suspended at this time but the virtual tranings via Zoom are provided (please contact Guowei Tian for detial). The trained users also must follow some additional procedures below:

(1). Make appointments on iLab calendar as you normally would at least in advance of one day but leave 1 hour or more gap time between users for disinfecting the scope;

(2). Must wear (a) a face mask and (b) fresh gloves all the time when using the scope;

(3). Only one user is allowed in microscope room at one time.

Thank you for your support and cooperation.

 

 

Overview of Services

 

CMIC at Stony Brook University is a core service center maintained by the School of Medicine’s

Office of Scientific Affairs (OSA). It provides two-photon confocal microscopy, super-resolution

microscopy, and transmission electron microscopy (TEM) services for internal and external communities.

 

CMIC includes two facilities operating independently:  1. Multiphoton Imaging facility, 2. Transmission Electron Microscope (TEM) facility.

 

 

1. Multiphoton Imaging Instrumentation

 

    (1). Zeiss LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System

          Inverted 2-photon confocal microscope capable of FRAP, FLIP, FRET, photoactivation, and spectral imaging. Equipped

          with incubator chamber for CO2 and temperature control for live cell imaging. Laser lines: 458nm, 477nm, 488nm,

          514nm, 543nm, 633nm, and Chaneloen laser tunable 705nm-980nm.

 

   (2). Olympus BX61WI In-Vivo Two-Photon Confocal Microscope

          Upright 2-photon confocal microscope with a custom-made stage capable of small live animal imaging. Laser line:

          Coherent Chameleon laser tunable 705nm-980nm.

 

   (3). Nikon N-STORM Super Resolution Microscope System

          N-STORM can produce 10 times the resolution of conventional optical microscopes by combining “Stochastic Optical

          Reconstruction Microscopy” (STORM) technology and Nikon's Eclipse Ti research inverted microscope, with lateral

          resolution to approximately 20nm and axial resolution to approximately 50nm, extending the role of the optical

          microscope to near molecular level resolution. 3D capable. Laser lines: 405nm, 488nm, 561nm, 647nm.

          STORM Protocol-Sample Preparation


   (4). Nikon N-SIM Super Resolution Microscope System

         N-SIM can produce nearly two times the resolution of conventional optical microscopes by combining “Structured

         Illumination Microscopy” (SIM) technology and Nikon Eclipse Ti research inverted microscope, with lateral resolution

         to approximately 100nm and axial resolution to approximately 200nm. 3D capable. TIRF capable. Laser lines: 405nm,

         488nm, 561nm, 640nm.

 

   (5). Two Workstations

         One standalone workstation with Nikon NIS-Element (Ar) software, STORM images, and SIM images off-line analysis

         licenses.

 

         One standalone workstation with Zeiss LSM software (version 4.0) off-line image analysis license

     

   (6). Others (preparative equipment)

  • Class II Biological Safe Cabinet, Baker Company
  • CO2 Incubator, SANYO
  • 4 Co Refrigerator, SANYO

 

2. TEM Instrumentation

 

      (1). FEI BioTwinG2 Transmission Electron Microscope-acceleration voltage of 120kV with goiniometer/stage tilt capability.
      (2). AMT XR-60 CCD digital camera system for acquiring digital images as well as film capability.
      (3). Leica EM UC7 Ultramicrotome.

 

  Sample Preparation

      (1). Routine sample preparation (embedding sectioning and staining)
      (2). Immunohistochemistry using immunogold techniques
      (3). Negative staining
      (4). Serial Sectioning

 

Leadership

 

Lina M. Obeid, MD, Vice Dean for Research

 

Glen Itzkowitz, Associate Dean, Office of Scientific Affairs

 

Aaron Neiman, Ph.D., Faculty Director, Department of Biochemistry & Cell Biology

 

Location and hours of operation

 

Hours

 

Location

 

9:00am to 5:00pm      

Monday through Friday

Central Microscopy Imaging Center (CMIC)
048 Life Science Building
Stony Brook University
Stony Brook, New York 11794-5200

Links and Resources

 

  1. CMIC Website
  2. Office of Scientific Affairs (OSA)
  3. FEI
  4. Nikon
  5. N Storm Sample Submission Protocol
  6. Leica Microsystems
  7. Zeiss
  8. ImageJ

 

Links to Software Tutorials

How to Log In

Using your Home Page

Managing Lab Settings (for Lab Managers and PIs)

Making Service Requests

Making Equipment Reservations

Contacts

Name Role Phone Email Location
Guowei Tian (Multiphoton Imaging)
Facility Manager
 
Guowei (631) 632-9331
 
Guowei.Tian@stonybrook.edu
 

 
Susan C. Van Horn
TEM Facility Manager
 
Sue (631) 632-8623
 
susan.vanhorn@stonybrook.edu